Adenovirus Control Prodcution
Introduction to Adenovirus Control Prodcution
Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. Recombinant protein expression of interest is detectable from 24 hours post infection.
Genemedi has launched a variety of control adenovirus encoding GFP, RFP and luciferase. When a new experiment is beginning, it is necessary to test the infection efficiency with control adenovirus at first to find a suitable MOI for the target cells.
Properties
| Adenovirus Vector | |
|---|---|
| Quantity/Unit | Vials. |
| Form | Frozen form. |
| Sipping and Storage Guidelines | Shipped by dry ice, stored at -80 ° C, effective for 1 year. Avoid repeatedly freezing and thawing. |
| Titer | > 1*10^10 PFU/ml. |
Advantages
1. Broad range of host. Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.
2. High infection efficiency. Almost 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection.
3. Great packaging capacity, up to 8kb.
4. Without integration. No alteration to host genome.
5. High titer. Up to 1011 pfu/ml.
Applications and Figures
Quality control description
Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.
Technical Documents
1. For detailed protocol about how to package and purify Adenovirus, please see the Adenovirus User Manual on the Genemedi website.
Reference
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