Adenovirus protocol Download Adenovirus Production Protocol–Genemedi 1. Adenovirus plasmid construction The gene of interest is cloned into one of the ITR/MCS-containing adenovirus vectors to generate pAd -GOI. The purity and RNA contaminants of viral plasmid should be taken into consideration. 2. Adenovirus packaging The recombinant adenovirus viral plasmid pAd-GOI is co-transfected into the 293A with […]
Adenovirus protocol Download Adenovirus Infection Protocol in vitro and in vivo a. Adenovirus Infection Protocol–Genemedi For the reason that MOI varies in different cell lines, preliminary experiment is necessary to ensure a proper MOI of target cells before conducting formal experiments.Note: MOI, multiplicity of infection, is the number of viral particles to infect one cell. […]
Adenovirus protocol Download Custom Lentivirus Production Services Custom Lentivirus Production Service
Adenovirus protocol Download For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR) [17]. Upon attachment, interaction between the penton-base Arg-Gly-Asp (RGD) and cellular αv integrins, which can stimulate actin polymerization, leads to internalization of the virus into […]
Adenovirus protocol Download Adenovirus is a non-enveloped, 90-100 nm diameter virus presenting icosahedral symmetry (Figure1A). Human adenovirus, containing a linear, double-stranded DNA genome, which is approximately 36kb, is wrapped in a histone-like protein and has two inverted terminal repeats (ITRs) of 50-200 bp, which act as origins of replication, accompanied by a complex series of […]
Adenovirus protocol Download a) Advantages of Adenovirus-mediated gene delivery Adenovirus has been developed into a preferred candidate for creating viral vectors for gene therapy due to various advantages.1) Well tolerated, with post-infection viability of the host cells being almost 100%.2) Great packaging capacity (up to 8kb).3) Broad range of infectivity. Adenovirus can infect both dividing […]
Adenovirus protocol Download Adenovirus has been proved as an excellent gene therapy vector. To date, more than 535 clinical trials have been carried out using adenovirus vectors for gene delivery [7], and promising gene therapy outcomes from recombinant adenovirus have been achieved from clinical trials for a great number of diseases (Table 1), especially for […]
Adenovirus protocol Download Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [1]. It is a medium-sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a double-stranded DNA genome. With a broad range of vertebrate hosts, 100 serotypes have been isolated, and […]
AAV protocol Download AAV Production Protocol–Genemedi 1. AAV plasmid construction The gene of interest is cloned into one of the ITR/MCS-containing AAV vectors to generate AAV-GOI. The purity and RNAse contaminants of AAV viral plasmid should be taken into consideration. 2. AAV packaging The recombinant AAV viral plasmid AAV-GOI is co-transfected into the AAV-293 cells […]
AAV protocol Download Adeno Associated Virus (AAV) For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is […]
