AAV protocol Download Adeno Associated Virus (AAV) AAV transduces cells through several stages: (1) viral binding to cell surface receptor/coreceptor, (2) endocytosis of the virus, (3) intracellular trafficking of the virus through the endosomal compartment, (4) endosomal escape of the virus, (5) intracellular trafficking of the virus to the nucleus and nuclear import, (6) virion […]
AAV protocol Download Adeno Associated Virus (AAV) The AAV shell is approximately 25nm in diameter and encapsidates a single-stranded DNA genome of 4.7 kilobases. The genome consists of two large open reading frames (ORFs) flanked by 145bp inverted terminal repeats (ITR), which are the only cis-acting elements required for AAV genome replication and AAV packaging. […]
AAV protocol Download Adeno Associated Virus (AAV) a) Advantages of AAV-mediated gene transferAAV has been developed into a very attractive candidate for creating viral vectors for gene therapy and the creation of isogenic human disease models due to various advantages. 1) Superior biosafety rating. The wild type AAV has not currently been known to cause […]
AAV protocol Download Adeno Associated Virus (AAV) AAV has been proved as the most excellent gene therapy vector. To date, more than 204 clinical trials have been carried out using AAV vectors for gene delivery [4], and promising gene therapy outcomes have been achieved from Phase 1, Phase 2 and Phase 3 trials for a […]
AAV protocol Download Adeno Associated Virus (AAV) Adeno-associated virus (AAV) is a small-single strand DNA virus, member of human parvovirus, originally described in the 1960s by Bob Atchison at Pittsburgh and Wallace Rowe at NIH as a contaminant in preparation of adenovirus [1,2]. Shortly after its discovery, AAV was then isolated from humans, but serological studies […]
AAV(adeno associated virus) User Manual AAV-LC3 User Manual AAV-SaCas9 User Manual
AAV protocol Download AAV Production Protocol–Genemedi 1. AAV plasmid construction The gene of interest is cloned into one of the ITR/MCS-containing AAV vectors to generate AAV-GOI. The purity and RNAse contaminants of AAV viral plasmid should be taken into consideration. 2. AAV packaging The recombinant AAV viral plasmid AAV-GOI is co-transfected into the AAV-293 cells […]
AAV protocol Download Adeno Associated Virus (AAV) For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is […]
AAV protocol Download AAV capsids evolution and tissue-specific AAV discovery & development service The Genemedi help scientists and researchers in gene therapy vector development. Besides different scales of AAV production, we can also offer novel AAV discovery services with AAV re-engineering capsids library and sub-library generation based on different strategies, customized AAV capsids screening and […]
Adeno-associated virus (AAV) Knowledge base AAV serotypes and AAV Tissue-specific Tropism Over the past decades, numerous AAV serotypes have been identified with variable tropism. Read More Life Cycle of AAV Virus-From Infection To Regeneration AAV establishes latency by undergoing specifically integration into a genome site, termed as the adeno-associated virus integration site 1 (AAVS1), a […]
