Lentivirus Protocol Download This protocol is for the stable cell line construction based on puromycin selection. Day 1: Seed target cells in 24-well plates. The number of seeding cells differs according to the cell proliferation rate.Day 2: Target cells should be approximately 50%-70% confluent. For polybrene accessible cells, mix the culture medium with proper concentrations of polybrene. […]
AAV protocol Download Adeno Associated Virus (AAV) For normal tissues or organs, such as heart, liver, kidney, breast, pancreas, ovary, brain, eye, skeleton muscle, adipose tissue, etc., Genemedi systematically organizes the corresponding optimal AAV serotype, gene delivery method and injection volume for mouse and rat tissue infection. Recommended AAV gene delivery methods for different organs is […]
Lentivirus Protocol Download a) Introduction of recombinant lentivirus vector system Since wild-type HIV-1 based lentivirus is associated with destruction of host immune system, especially CD4+ helper T lymphocytes, multiple generations of lentivirus vectors have been designed with enhanced safety features and as attractive vectors for gene therapy. To date, there have been three generations of […]
AAV protocol Download AAV capsids evolution and tissue-specific AAV discovery & development service The Genemedi help scientists and researchers in gene therapy vector development. Besides different scales of AAV production, we can also offer novel AAV discovery services with AAV re-engineering capsids library and sub-library generation based on different strategies, customized AAV capsids screening and […]
Lentivirus Protocol Download The life cycle of HIV-1 starts with viral entry, in which process the virus binds to the CD4 receptor or a coreceptor (CCR5 or CXCR4) with gp120 protein, thereby anchoring itself onto host cell surface, allowing fusion between the cellular and viral membranes. After entry into the cell, the viral nucleoprotein together […]
CRISPR/Cas9 premade products Custom made CRISPR/Cas9 service Custom-made CRISPR/Cas9-gRNA lentivirusfor knockout cell line development Custom-made CRISPR/Cas9-gRNA adenovirusfor knockout cell line development Custom-made CRISPR/Cas9-gRNA AAVfor tissue-specific knockout in vivo CRISPR/Cas9 User Manual Crispr/cas9 mediated Gene knockout AAV Production CRISPR/Cas9Knockout System-User Manual Adenovirus CRISPR/Cas9Knockout System-User Manual Lentivirus-CRISPR/Cas9Knockout System-User Manual Introduction and principles of […]
Adenovirus Knowledge base What is Adenovirus vector? Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [1]. It is a medium-sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a double-stranded DNA genome. Read More Adenovirus Gene Therapy Review Adenovirus has been […]
Lentivirus Knowledge base What is Lentivirus Lentivirus (lente-, Latin for “slow”) is a genus of retroviruses, causing chronic and deadly diseases by long incubation periods in human or other mammalian species [1]. The virion is a medium-sized (80-100nm) and enveloped, slightly pleomorphic, spherical with an isometric nucleocapsid containing two copies of positive-sense ssRNA genome. Read […]
Adeno-associated virus (AAV) Knowledge base AAV serotypes and AAV Tissue-specific Tropism Over the past decades, numerous AAV serotypes have been identified with variable tropism. Read More Life Cycle of AAV Virus-From Infection To Regeneration AAV establishes latency by undergoing specifically integration into a genome site, termed as the adeno-associated virus integration site 1 (AAVS1), a […]
The Genemedi help scientists and researchers in gene therapy vector development. Besides different scales of AAV production, we can also offer novel AAV discovery services with AAV re-engineering capsids library and sub-library generation based on different strategies, customized AAV capsids screening and tissue-specific AAV tropism validation. 1. AAV engineering capsids library and sub-library generation 1) […]
