Lentivirus Protocol Download Lentivirus Production Protocol–Genemedi 1. Lentivirus plasmid construction The gene of interest is cloned into one of the LTR/MCS-containing lentivirus vectors to generate pLV-GOI. The purity and RNA contaminants of viral plasmid should be taken into consideration. 2. Lentivirus packaging The recombinant lentivirus viral plasmid pLV-GOI is co-transfected into the 293T with envelope […]
Category Archives: lentivirus
Lentivirus Protocol Download This protocol is for the stable cell line construction based on puromycin selection. Day 1: Seed target cells in 24-well plates. The number of seeding cells differs according to the cell proliferation rate.Day 2: Target cells should be approximately 50%-70% confluent. For polybrene accessible cells, mix the culture medium with proper concentrations of polybrene. […]
Lentivirus Knowledge base What is Lentivirus Lentivirus (lente-, Latin for “slow”) is a genus of retroviruses, causing chronic and deadly diseases by long incubation periods in human or other mammalian species [1]. The virion is a medium-sized (80-100nm) and enveloped, slightly pleomorphic, spherical with an isometric nucleocapsid containing two copies of positive-sense ssRNA genome. Read […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay Table of Contents Abstract The vaccine helps to provoke the immune system and is an efficacious means for disease prevention and treatment. At this particular time of the COVID-19 outbreak, the vaccine for COVID-19 is urgently needed to save tens of thousands of people’s lives. Here we […]
GeneMedi Lab offer a variety of Pre-made and Pre-packaging control lentiviral vector (lentivirus with vsv-G envelop) particleswith different fluorescent labels (such as GFP, RFP, YFP, luciferase, zsgreen, mCherry and so on), GeneMedi’s lentiviral vector drived by various promoters (such as CMV, CAG, U6, syn, TBG and cTNT etc.) with antibiotics resistance (for example, puromycin resistance). […]
