Protocol Download 1. Dissolve 5mg HRP in 1ml distilled water. 2. Add 200 μL of 0.1M NalO4 solution [1] (freshly prepared just before use) to the solution obtained in step 1 and stirred for 20 minutes at room temperature. Protect from light. 3. Put the solution obtained in step 2 into a dialysis bag and dialyzed with […]
Category Archives: protocol
Protocol Download 1. Recombinant human ACE2 was diluted to a concentration of 5μg/ml with 0.05 M PH 9.6 carbonate buffer [1]. Then add 100 μL to each well of polystyrene plate at 4°C overnight. 2. Aspirate the solution in the well and wash each well three times with Wash Buffer[2]. Complete removal of liquid at each […]
SARS-CoV-2 (2019nCoV) pseudotype virus (pseudovirus, PSV) for COVID-19 related vaccines and neutralizing antibodies evaluation. The outbreak of COVID-19, caused by SARS-CoV-2 (2019-nCoV), has been a global public health threat and caught the worldwide concern. Due to its high pathogenicity and infectivity1, live SARS-CoV-2 should be handled under biosafety level 3 (BSL-3) conditions. GeneMedi has developed […]
- 1
- 2
