ClonEasyTM?One Step Cloning Kit
Cat:R-GC
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Introduction to ClonEasyTM?One Step Cloning Kit
Genemedi ClonEasyTM One Step Cloning Kit allows for the joining of multiple DNA fragments in a single, isothermal reaction, regardless of fragment length or end compatibility. The method can simultaneously combine up to 15 DNA fragments based on sequence identity. It requires that the DNA fragments contain ~20-40 base pair overlap with adjacent DNA fragments. The great part about this mix of enzymes is that they can all work at the same temperature, so the entire reaction takes an hour or less to complete at 50 °C. After an hour or so, the sample is immediately ready to transform into competent cells. Thus, it has been rapidly adopted by the synthetic biology community.
The end-result is a double-stranded fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation.
Properties
Quantity/Unit
1 kit.
Form
Liquid.
Sipping and Storage Guidelines
Shipped at 4°C. Recommended storage at -20°C, effective for 2 years.
Suitable Cell Type
Sample Type
Serum Compatible
Advantages
1. Fewer steps. One tube reaction.2. No scar between joined fragments.3. No PCR clean-up step required.4. Can combine many DNA fragments at once.5. Generate wild type and mutant constructs at the same time.6. High transformation efficiencies for inserts up to 20 kb.7. Easily adapted for multiple DNA manipulations, including site-directed mutagenesis.
