Diagnostic anti-neosporidium canis antibodies pairs and antigens for animal health (animal infectious disease ) testing in ELISA test,competitive ELISA test, blocking ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), and immunonephelometry

Neosporidium canis (Neospora caninum) is a kind of pathogenic protozoa that parasitize the host cells of lactation. It can infect most mammals, among which the most serious harm to cattle. Beef cattle or cows infected with N.caninum mainly cause abortion and bring huge economic losses to cattle industry all over the world. However, the mechanism of N.caninum cell infection has not been fully clarified. In particular, there are few reports on the regulation of host signal transduction and cell survival or death by N.caninum. EGFR (epidermal growth factor receptor) pathway is an important signal pathway involved in the regulation of cell survival. It has been reported that Toxoplasma gondii can inhibit the apoptosis of infected cells and that Toxoplasma gondii infection activates EGFR-AKT signaling pathway and inhibits the target and killing effect of autophagy protein on infected cells. However, in Neosporidium, whether the infection can regulate the EGFR signal is less reported, whether the EGFR signal transduction can control the apoptosis of host cells is still unknown. Therefore, in this paper, we will make a detailed study of the molecular mechanism of N.caninum on the host EGFR signal transduction pathway and apoptosis regulation. The thesis includes the following parts: 1. After stimulation with neosporidium, the expression of EGFR signaling protein and apoptosis-related protein in host was detected. The results showed that N.caninum infection could activate the EGFR,EGFR-AKT and EGFR-MAPK signaling pathway of host rapidly, and then regulate the expression of apoptosis-related protein. 2. The effect of Neosporidium infection on cell viability and apoptosis was evaluated by detecting apoptosis-related indexes. The results showed that N.caninum infection could inhibit apoptosis in early stage, and its inhibition was mainly mediated by activation of EGFR-AKT pathway. The molecular mechanism of neosporidium activating EGFR signal transduction and inhibiting cell apoptosis was discussed. The results showed that N.caninum could activate host EGFR signal transduction through interaction between EGF-MIC and host cell surface EGF receptor, and then inhibit cell apoptosis; 4. The effects of siRNA on Neosporidium cell infection were detected after blocking and interfering the host EGFR pathway with specific inhibitors. The results showed that after inhibiting the activation of EGFR signaling pathway or silencing EGFR,AKT, the cell infection of Neosporidium was significantly inhibited. In conclusion, this study discussed the molecular mechanism of neosporidium regulating cell apoptosis. The results showed that N.caninum infection could inhibit the apoptosis of host cells. The inhibition of apoptosis is mediated by the EGF-like domain EGF-MIC, which mediates the activation of the EGFR-AKT signaling pathway by interacting with the host EGFR. And further regulate the expression of apoptosis-related proteins, resulting in the inhibition of cell apoptosis effect. These new information will help us to further understand the function of EGFR signal transduction in the process of parasite infection and the cell infection and survival mechanism of Neosporidium. It provides a theoretical basis for neosporidium to participate in the regulation of host function, and it is of great significance to find and design effective drugs for chemical intervention of neosporidiosis in the future.Genemedi produces core animal health diagnostic ingredients-validated antibodies pairs Mouse anti-neosporidium canis monoclonal antibody and neosporidium canis antigens for rapid test kit of animal infectious disease with neosporidium canis to evaluate the animal health of Pet.The paired antibodies are both monoclonal antibody(mab).
All the antibodies and antiges of animal disease test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibody can act as a capture antibody and detection antibody. Antigens are validated as positive control materials.