AAV8-1m vector system (retina-validated AAV expression and packaging plasmids)

GeneMedi’s AAV6 Vector System (AAV serotype 6 helper-free packaging plasmids system) is including AAV6 Rep-Cap plamid (AAV6-RC plasmid, or called AAV-RC6 plasmid), AAV helper plasmid and AAV expression vectors (overexpression or shRNA).

GeneMedi’s AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi’s AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA AAV expression vector.

AAV6 Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV6 capsid protein.

The tissue tropism of AAV6 vector has been validated in neuron (CNS), lung, liver, heart and muscle, with potential applications in tissue-specific gene therapy.

AAV6 has also been proved a high transduction efficency in T cell, wtih potential applications in T cell-mediated cancer immunotherapies, including Chimaeric antigen receptor T(CAR-T) and TCR engineered T (TCR-T) cell therapy.

AAV6 vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV6 vector has been validated in neuron (CNS), lung, liver, heart and muscle, with potential applications in tissue-specific gene therapy.

AAV6 has also been proved a high transduction efficency in T cell, wtih potential applications in T cell-mediated cancer immunotherapies, including Chimaeric antigen receptor T(CAR-T) and TCR engineered T (TCR-T) cell therapy.

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×10¹² vg/ml
Cells: HEK-293T
MOI: MOI=1×10⁴
Determine assay: 36 hours post infection, immunofluorescence microscopy