AAV2 vector system (AAV2 expression system, AAV2 packaging plasmid system)

GeneMedi’s AAV2 Vector System (AAV serotype 1 helper-free packaging plasmids system) is including AAV2 Rep-Cap plamid (AAV2-RC plasmid,or called AAV-RC1 plasmid), AAV helper plasmid and AAV expression vectors (overexpression or shRNA).

GeneMedi’s AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi’s AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA AAV expression vector.

AAV2 Rep-Cap plamid supplies the AAV2 Rep(replication) proteins and the AAV2 capsid protein.

The tissue tropism of AAV2 vector has been validated in retina, liver and kidney, with potential applications in tissue-specific gene therapy.

AAV2 vector tissue tropism and gene transduction (serotype-specific AAV infection)

The tissue tropism of AAV2 vector has been validated in retina, liver and kidney, with potential applications in tissue-specific gene therapy.

Virus and titer: AAV2 (left) /AAV-DJ (middle) / AAV9 (right), 1×10¹² vg/ml
Animal: Nude mouse, 2 months
Infection site: Subcutaneous transplant of bowel cancer cells
Gene delivery method: Tumor injection, 10μl/site, 4 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy