Author Archives: lwj

aav, gene therapy

AAV FAQs

Posted on by lwj

How can I choose the optimal AAV serotypes for my in vivo study? Genemedi has launched a comprehensive AAV production service. More than 12 AAV serotypes and a variety of capsid engineered AAV vectors are available for targeting different tissues and organs. Different AAV serotypes have different tissue tropism in vivo, the common serotypes and […]

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gene therapy, lentivirus, protocol

Lentivirus Infection Protocol for stable cell line development (CLD)

Posted on by lwj

Lentivirus Protocol Download This protocol is for the stable cell line construction based on puromycin selection. Day 1: Seed target cells in 24-well plates. The number of seeding cells differs according to the cell proliferation rate.Day 2: Target cells should be approximately 50%-70% confluent. For polybrene accessible cells, mix the culture medium with proper concentrations of polybrene. […]

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