Author Archives: shanzhu

TCR-T immunotherapy and cancer therapy

Lentivirus Protocol Download Cell therapy is a kind of medicine aiming to cure disease or alleviate disease symptoms via direct infusion or transplantation of cells, which can be autologous or allogeneic. With several decades’ development and optimization, immuno-oncology cells (such as T cells, nature killer cells, etc.), stem cells (embryonic stem cells, induced pluripotent stem […]

CAR-T immunotherapy and cancer therapy

Lentivirus Protocol Download Cell therapy is a kind of medicine aiming to cure disease or alleviate disease symptoms via direct infusion or transplantation of cells, which can be autologous or allogeneic. With several decades’ development and optimization, immuno-oncology cells (such as T cells, nature killer Chimeric antigen receptor (CAR)-modified T cells (CAR-T) are T cells […]

Diagnostic antibodies and antigens for functional ELISA assay kit, lateral flow and other immunoassays in diagnostics

Diagnostic antibodies and antigens list In vitro diagnostic (IVD) testing has become an indispensable tool in clinical practice for diagnosing and monitoring of diseases, as well as providing prognosis and predicting treatment response, In vitro tests may be done in laboratories, health care facilities or even in the home. The tests themselves can be performed […]

Preparation of AuNP conjugates with recombinant antigens, protein or antibodies for immunoassay

Protocol Download 1. To prepare the AuNP (gold nanoparticle) conjugate, the recombinant protein dissolved in PBS (1 mg/mL) was added to the mixture of 1mL AuNP colloid (40nm in diameter, OD=1) and 0.1mL of borate buffer (0.1 M, pH 8.5). Incubation for 30 min at room temperature. 2. 0.1mL of 10mg/mL BSA in PBS was […]

Protocol of SARS-CoV2 (2019nCoV) Spike-RBD antibodies standard biopanning and competitive biopanning

Protocol Download Standard biopanning 1. S-RBD was diluted with 0.05 M PH 9.6 carbonate buffer [1]. Then add 100 μL to each well of 96-well Maxisorp plates at 4°C overnight. 2. Aspirate the solution in the well and wash each well three times with Wash Buffer [2] (300 μL/well). Complete removal of liquid at each step is essential […]

Protocol of SARS-CoV2 (2019nCoV) NP paired antibodies sandwich ELISA

Protocol Download 1. The Capture Antibody #1 was diluted to a concentration of 1 ~ 10 ug/ml with 0.05 M PH 9.6 carbonate buffer [1]. Then add 100 μL to each well of polystyrene plate at 4℃ overnight. 2. Aspirate the solution in the well and wash each well three times with Wash Buffer [2] (300 μL/well). Complete removal of […]

Detection Antibody HRP labeling For diagnostics application (ELISA)

Protocol Download 1. Dissolve 5mg HRP in 1ml distilled water. 2. Add 200 μL of 0.1M NalO4 solution [1] (freshly prepared just before use) to the solution obtained in step 1 and stirred for 20 minutes at room temperature. Protect from light. 3. Put the solution obtained in step 2 into a dialysis bag and dialyzed with […]

Protocol of SARS-CoV-2 Pseudovirus (PSV)-Based Neutralization Assay For Vaccines, therapeutic antibodies, peptides and compounds against COVID-19

SARS-CoV-2 (2019nCoV) pseudotype virus (pseudovirus, PSV) for COVID-19 related vaccines and neutralizing antibodies evaluation. The outbreak of COVID-19, caused by SARS-CoV-2 (2019-nCoV), has been a global public health threat and caught the worldwide concern. Due to its high pathogenicity and infectivity1, live SARS-CoV-2 should be handled under biosafety level 3 (BSL-3) conditions. GeneMedi has developed […]

Diagnostic antibodies and antigens for food safety testing in mycotoxin and chemical contamination

Full List Download Competitive immunoassay-validated anti-Hapten (small molecules, chemicals) antibody against hapten (small molecules, chemicals) and hapten-carrier conjugated competitive antigens(Carrier-coupled antigen,immunogen,hapten-carrier conjugates, BSA-conjugated, OVA-conjugated). The anti-Hapten antibodies against haptens had been validated with our hapten-carrier conjugates via competitive ELISA test. GeneMedi offers paired anti-small molecules (Chemicals, Antibiotics,Mycotoxins,Hormones,Drugs of Abuse, etc.) antibodies (monoclonal antibody, mab) and […]