Adenovirus vector -Introduction Landscape, protocol and guidelines of adenovirus vector system (adeasy and admax), adenovirus production, adenovirus mediated gene transduction in vitro and in vivo are described below. The detail information is mentioned about adenoviral vector (adenovirus plasmids) cloning, adenovirus packaging, purification and the insight of adenovirus gene delivery and gene therapy) Adenovirus vector -Index […]
1. Why do you offer GeneGelRed in DMSO or water? The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some […]
1. What is the maximum length of the DNA fragment assembled? Gibson Assembly Cloning Kit has been used to clone a 15 kb DNA fragment into a 5 4 kb plasmid in E. coli, totaling up to 20 4 kb in length 2. How many fragments of DNA can I assemble in one reaction? The […]
1. What is the recommended dilution range for Femto Signal Western ECL Substrate? Primary antibody recommended dilution range is 1 to 0.2 µg/mL primary antibody (1:5,000 to 1:100,000 dilution from 1µg/mL stock). Secondary antibody recommended dilution range is 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from µg/mL stock). 2. What is the […]
1. What is the recommended dilution range for Hypersensitive ECL chemiluminescence Kit? Primary antibody recommended dilution range is 0.2 to 1.0 µg/mL (1:1000 to 1:5000 dilution from a 1 mg/mL stock). Secondary antibody recommended dilution range is 10 to 50 ng/mL (1:20,000 to 1:100,000 dilution from 1 mg/mL stock). 2. What is the sensitivity of […]
1. How many cells should there be in a well? For adhesive cells, at least 1000 cells are required per well (100 μl medium) when using the kit’s standard 96-well plate. For leukocytes, at least 2500 cells are necessary per well (100 μl medium) because of low sensitivity. The recommended maximum number of cells per […]
1. What are Mycoplasmas? Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes. They are flexible, pleomorphic organisms that can be as small as 0.2 to 0.3. 2. What are the consequences of mycoplasma contamination? Because of their typically high concentrations in cell cultures, mycoplasmas can often […]
Q1. Are cell density (% confluence) and passage number important considerations for transfection? A: Yes, cell density is an important parameter in influencing transfection efficiency. If the seeding density is too low, some cytotoxicity may be observed. If the cell density is high, lower than expected transfection efficiency may be observed. Both issues may be […]
1. Why does adenovirus have a relatively higher immunogenecity compared with rAAV? The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. 2. What is the role of the […]
AAV FAQs Lentivirus FAQs Adenovirus FAQs Lipogene™ Transfection reagent FAQs Mycoplasma Elimination Reagent FAQs Cell Counting Kit-8 FAQs Pico Signal Western ECL Substrate FAQs Femto Signal Western ECL Substrate FAQs ClonEasy™ One Step Cloning Kit FAQs GeneGelRed™ Nucleic Acid Gel Stain FAQs Experiment general FAQs
