1. What is the recommended dilution range for Femto Signal Western ECL Substrate? Primary antibody recommended dilution range is 1 to 0.2 µg/mL primary antibody (1:5,000 to 1:100,000 dilution from 1µg/mL stock). Secondary antibody recommended dilution range is 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from µg/mL stock). 2. What is the […]
1. What is the recommended dilution range for Hypersensitive ECL chemiluminescence Kit? Primary antibody recommended dilution range is 0.2 to 1.0 µg/mL (1:1000 to 1:5000 dilution from a 1 mg/mL stock). Secondary antibody recommended dilution range is 10 to 50 ng/mL (1:20,000 to 1:100,000 dilution from 1 mg/mL stock). 2. What is the sensitivity of […]
1. How many cells should there be in a well? For adhesive cells, at least 1000 cells are required per well (100 μl medium) when using the kit’s standard 96-well plate. For leukocytes, at least 2500 cells are necessary per well (100 μl medium) because of low sensitivity. The recommended maximum number of cells per […]
1. What are Mycoplasmas? Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes. They are flexible, pleomorphic organisms that can be as small as 0.2 to 0.3. 2. What are the consequences of mycoplasma contamination? Because of their typically high concentrations in cell cultures, mycoplasmas can often […]
Q1. Are cell density (% confluence) and passage number important considerations for transfection? A: Yes, cell density is an important parameter in influencing transfection efficiency. If the seeding density is too low, some cytotoxicity may be observed. If the cell density is high, lower than expected transfection efficiency may be observed. Both issues may be […]
1. Why does adenovirus have a relatively higher immunogenecity compared with rAAV? The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. 2. What is the role of the […]
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Selected Publications using Genemed’s AAV Products/Services Publications Link to the article Products/Services H. Zhu, et. al, ‘Moderate Uv Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain’, Cell, 173 (2018), 1716-27 e17 http://www.ncbi.nlm.nih.gov/pubmed/29779945 AAV virus H. Yang et. al, ‘Laterodorsal Tegmentum Interneuron Subtypes Oppositely Regulate Olfactory Cue-Induced Innate Fear’, […]
Abstract The clinical translation of cationic α-helical antimicrobial peptides (AMPs) has been hindered by structural instability, proteolytic degradation and in vivo toxicity from nonspecific membrane lysis. Although analyses of hydrophobic content and charge distribution have informed the design of synthetic AMPs with increased potency and reduced in vitro hemolysis, nonspecific membrane toxicity in vivo continues […]
Abstract Ovarian cancer and triple-negative breast cancer are among the most lethal diseases affecting women, with few targeted therapies and high rates of metastasis. Cancer cells are capable of evading clearance by macrophages through the overexpression of anti-phagocytic surface proteins called ‘don’t eat me’ signals—including CD471, programmed cell death ligand 1 (PD-L1)2 and the beta-2 […]
