Transfection protocol Download Introduction of transfection In general, transfection is the process of delivering nucleic acids, such as DNA and RNA into eukaryotic cells, resulting in the expression or production of proteins or down-regulation of the targeted protein. Although not very common, protein transfection is also used to promote the rapid expression of target protein […]
Category Archives: gene therapy article
Amino Acid Structure Chart and Reference Table Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains. The properties of amino acids are determined by the functional substituents linked on the side chains, which are most commonly referred to as R […]
Amino Acid Structure Chart and Reference Table GenScript offers a variety of peptide synthesis services with both natural and non-standard/unnatural amino acids. To order our Custom Peptides and Peptide Library services through our online ordering system, refer to the following list of amino acid codes below. You can use either the single or multiple letter […]
How much do you trust Dr. Google? Date:November 28, 2018 Source:University of Surrey Summary:Women experiencing signs of breast cancer vary in how they value, use, and trust ‘Dr. Google’ when making sense of their symptoms, a new study reports. Women experiencing signs of breast cancer vary in how they value, use, and trust ‘Dr Google’ […]
Weather forecasting is the application of current technology and science to predict the state of the atmosphere for a future time and a given location. Weather forecasts are made by collecting as much data as possible about the current state of the atmosphere (particularly the temperature, humidity and wind) and using understanding of atmospheric processes […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay 5. Vaccines development for COVID-19/SARS-CoV-2 Coronavirus Disease 2019 (COVID-19) is a novel viral pneumonia caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). First discovered in Wuhan, a city in Hubei province of China, COVID-19 has already broken out throughout the world and posed a great threat […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay 4. RNA based vaccines Though DNA vaccines avoid the anti-vector immunity, long term existence in cells might produce excessive antigens, which might provoke overactive immune responses and exhaust T cells [140]. Thus, another kind of nucleic acid-based vaccines, RNA vaccines, were developed by directly delivering mRNA into […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay Comparison of commonly used viral vector-based vaccines Viral vector-based vaccines can provoke innate and adaptative immune responses in host system besides the transgene antigens, which makes them preferred during vaccine design and development. Nevertheless, the immune responses induced by different viral vectors may have some differences (Table […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay Lentivirus (lente-, Latin for “slow”) is a genus of retroviruses, medium sized (80-100nm) and enveloped, slightly pleomorphic, spherical with an isometric nucleocapsid containing two copies of positive-sense ssRNA genome (Fig. 11A). Most lentiviral vectors are based on the Human Immunodeficiency Virus (HIV), which causes AIDS, a chronic […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay Adenovirus (AdV) vector-based vaccines Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. It is a medium sized (90-100nm) and non-enveloped virus with an icosahedral nucleocapsid containing a 36kb double stranded DNA genome (Fig. […]
