Category Archives: gene therapy

AAV vector system (AAV expression system, AAV packaging plasmid system )

AAV protocol Download AAV User Manual Download Introduction to AAV Vector System (AAV packaging and expression system) GeneMedi’s AAV Vector System (AAV helper-free packaging system) including AAV expression vectors, AAV helper plasmid, and kinds of AAV Rep-Cap plamids (AAV-RC) with different serotypes of AAV capsids containing AAV2, AAV5, AAV6, AAV8, AAV9, AAV-PHP.B, AAV-PHP.eB, AAV PHP.s, […]

CRISPR/Cas9 Knockout Adenovirus Vectors

Introduction to CRISPR/Cas9 Knockout Adenovirus Vectors CRISPR/Cas9 premade products Custom made CRISPR/Cas9 service Custom-made CRISPR/Cas9-gRNA lentivirus for knockout cell line development Custom-made CRISPR/Cas9-gRNA adenovirusfor knockout cell line development Custom-made CRISPR/Cas9-gRNA AAVfor tissue-specific knockout in vivo CRISPR/Cas9 User Manual

Promise-ORF™viral CDNA library: Sequence-verified ORF/CDNA clones in lentivirus (lentiviral ORF clones), AAV, adenovirus and mammalian expression vectors

Promise-ORF™ GeneMedi’s Promise-ORF™ offers the larger collection of ORF/cDNA expression clones of human, mouse, rat and some other species. All ORFs expression clones in viral vectors (lentivirus, AAV and adenovirus) or mammalian expression vectors are sequences-verified. Using our Promise-ORF™ viral-ready expression vectors, you can easily promote your viral vectors packaging, or you can transfect your mammalian […]

Adenovirus-LC3 production service for Autophagy Flux Detection

Customized ADV production Introduction to AAV-LC3 production service for Autophagy Flux Detection Premade LC3 Autophagy Biosensors Products and user manual Adeno associated virus(AAV) AAV-GFP-LC3 Autophagy Biosensor AAV-mRFP-GFP-LC3 Autophagy Biosensor Adenovirus Adv-GFP-LC3 Autophagy Biosensor Adv-mRFP-GFP-LC3 Autophagy Biosensor Lentivirus Lv-GFP-LC3 Autophagy Biosensor Lv-mRFP-GFP-LC3 Autophagy Biosensor LC3 Autophagy Biosensors User Manual AAV-LC3 User Manual    Adenovirus-LC3 User Manual  […]

Adenovirus Amplification and purification Service

Begin your customized ADV production process Introduction to Adenovirus Amplification and purification Service The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adv packaging by Genemedi shows almost 100% gene delivery in most […]

Adenovirus Specific for Suspension Cells

Introduction to Adenovirus Specific for Suspension Cells The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adv packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein […]

Adenovirus vector system, Adenovirus production and transduction

Adenovirus vector -Introduction Landscape, protocol and guidelines of adenovirus vector system (adeasy and admax), adenovirus production, adenovirus mediated gene transduction in vitro and in vivo are described below. The detail information is mentioned about adenoviral vector (adenovirus plasmids) cloning, adenovirus packaging, purification and the insight of adenovirus gene delivery and gene therapy) Adenovirus vector -Index […]

Publication-Autophagy

Selected Publications using Genemed’s Autophagy Products/Services Publications Link to the article Products/Services T. Yu et.al, ‘Fusobacterium Nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy’, Cell, 170 (2017), 548-63 e16. http://www.ncbi.nlm.nih.gov/pubmed/28753429 Autophagy flux RFP-GFP-LC3 biosensor M. Liu et.al, ‘Sirt6 Deficiency Exacerbates Podocyte Injury and Proteinuria through Targeting Notch Signaling’, Nat Commun, 8 (2017), 413. http://www.ncbi.nlm.nih.gov/pubmed/28871079 […]

Publication-Lipogene

Selected Publications using Genemed’s LipoGene™ Transfection Reagent Publications Link to the article Products/Services X. Ma et.al, ‘Hippo Signaling Promotes Jnk-Dependent Cell Migration’, Proc Natl Acad Sci U S A, 114 (2017), 1934-39. http://www.ncbi.nlm.nih.gov/pubmed/28174264 LipoGene™ Transfection Reagent Y. Li et.al, ‘A Novel Epigenetic Aml1-Eto/Thap10/Mir-383 Mini-Circuitry Contributes to T(8;21) Leukaemogenesis’, EMBO Mol Med, 9 (2017), 933-49. http://www.ncbi.nlm.nih.gov/pubmed/28539478 […]

Publication-Lentivirus

Selected Publications using Genemed’s Lentivirus Products/Services Publications Link to the article Products/Services J. Nan et.al, ‘Tnfr2 Stimulation Promotes Mitochondrial Fusion Via Stat3- and Nf-Kb-Dependent Activation of Opa1 Expression’, Circ Res, 121 (2017), 392-410. http://www.ncbi.nlm.nih.gov/pubmed/28637784 Lentivirus J. Wu et.al, ‘Microrna-30 Family Members Regulate Calcium/Calcineurin Signaling in Podocytes’, J Clin Invest, 125 (2015), 4091-106. http://www.ncbi.nlm.nih.gov/pubmed/26436650 Lentivirus H. […]