Promise-ORF™ GeneMedi’s Promise-ORF™ offers the larger collection of ORF/cDNA expression clones of human, mouse, rat and some other species. All ORFs expression clones in viral vectors (lentivirus, AAV and adenovirus) or mammalian expression vectors are sequences-verified. Using our Promise-ORF™ viral-ready expression vectors, you can easily promote your viral vectors packaging, or you can transfect your mammalian […]
Selected Publications using Genemed’s Lentivirus Products/Services Publications Link to the article Products/Services J. Nan et.al, ‘Tnfr2 Stimulation Promotes Mitochondrial Fusion Via Stat3- and Nf-Kb-Dependent Activation of Opa1 Expression’, Circ Res, 121 (2017), 392-410. http://www.ncbi.nlm.nih.gov/pubmed/28637784 Lentivirus J. Wu et.al, ‘Microrna-30 Family Members Regulate Calcium/Calcineurin Signaling in Podocytes’, J Clin Invest, 125 (2015), 4091-106. http://www.ncbi.nlm.nih.gov/pubmed/26436650 Lentivirus H. […]
COVID-19 vaccine development protocol: PSV Based Neutralization Assay Lentivirus (lente-, Latin for “slow”) is a genus of retroviruses, medium sized (80-100nm) and enveloped, slightly pleomorphic, spherical with an isometric nucleocapsid containing two copies of positive-sense ssRNA genome (Fig. 11A). Most lentiviral vectors are based on the Human Immunodeficiency Virus (HIV), which causes AIDS, a chronic […]
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Lentivirus Protocol Download Lentivirus Production Protocol–Genemedi 1. Lentivirus plasmid construction The gene of interest is cloned into one of the LTR/MCS-containing lentivirus vectors to generate pLV-GOI. The purity and RNA contaminants of viral plasmid should be taken into consideration. 2. Lentivirus packaging The recombinant lentivirus viral plasmid pLV-GOI is co-transfected into the 293T with envelope […]
Lentivirus Protocol Download This protocol is for the stable cell line construction based on puromycin selection. Day 1: Seed target cells in 24-well plates. The number of seeding cells differs according to the cell proliferation rate.Day 2: Target cells should be approximately 50%-70% confluent. For polybrene accessible cells, mix the culture medium with proper concentrations of polybrene. […]
Lentivirus Protocol Download a) Introduction of recombinant lentivirus vector system Since wild-type HIV-1 based lentivirus is associated with destruction of host immune system, especially CD4+ helper T lymphocytes, multiple generations of lentivirus vectors have been designed with enhanced safety features and as attractive vectors for gene therapy. To date, there have been three generations of […]
Lentivirus Protocol Download The life cycle of HIV-1 starts with viral entry, in which process the virus binds to the CD4 receptor or a coreceptor (CCR5 or CXCR4) with gp120 protein, thereby anchoring itself onto host cell surface, allowing fusion between the cellular and viral membranes. After entry into the cell, the viral nucleoprotein together […]
Lentivirus Protocol Download Lentivirus, as represented by HIV-1, is a medium-sized (80-100nm) and enveloped, slightly pleomorphic, spherical virus with an isometric nucleocapsid (Figure1A). Unlike other retroviruses, HIV-1 is featured by a set of additional regulatory and accessory genes [11,12]. Its DNA genome, transcribed from HIV-1 ssRNA, is approximately 9.7 kb and contains 9 ORFs. Besides […]
Lentivirus Protocol Download a) Advantages of lentivirus -mediated gene delivery Lentivirus has been developed as an attractive candidate for creating viral vectors for gene therapy due to various advantages.1) Customized cloning for any other gene ORF expression, shRNA/miRNA and CRISPR/Cas9.2) No known immunogenic proteins generated.3) High titer. 108TU/ml or 109TU/ml lentiviral titer for cell line […]
