AAV-CMV-mRFP-GFP-LC3
Cat:GM-AAVPAU01
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Description
Catalog No.
GM-AAVPAU01
Product Name
AAV-CMV-mRFP-GFP-LC3
Production Scale
2E+11 vg ~ 1E+15 vg
AAV packaging Serotype:
AAV1
AAV2
AAV2-mu1
AAV2-mu2
AAV2-mu3
AAV2-7m8
AAV5
AAV6
AAV8 AAV8-1m
AAV8-2m
AAV8-3m
AAV9
AAV10
AAV-DJ
AAV-DJ/8
AAV-Retro AAV-PHP.B
AAV-PHP.A
AAV-PHP.eB
AAV-PHPS
Final Deliverables
0.2 mL, titer>1E+12 vg/mL ~ 50 mL, titer>1E+13 vg/mL
Lead Time(workday)
10~40
Quantity/Unit
Vials
Form
Frozen form
Shipping and Storage Guidelines
Shipped by dry ice, stored at -80 ℃, effective for 1 year. Avoid repeatedly freezing and thawing
Advantages
1. Real time and quantitative in vivo autophagy flux detection.
2. High resolution, more accuracy and sensitivity, than traditional approaches.
3. Safety. The wild type Adeno Associated Virus (AAV) has not currently been known to cause disease, and further security of recombinant AAV is ensured after removal of most AAV genome elements.
4. Low immunogenicity. AAV causes a very mild immune response, lending further support to its apparent lack of pathogenicity.
5. Broad range of host and specificity targeting. AAV has the ability to infect both dividing and quiescent cells, allowing genetic material to be delivered to a highly diverse range of cell types. More than 12 AAV serotypes and a variety of capsid engineered AAV vectors can be selected according to their tissue tropisms.
6. Stable physical properties. AAV is still alive at 60℃ and resistant to chloroform.
Applications and Figures
Quality control description
AAV capsid contains VR1 82kDa, VR2 72kDa and VR3 62kDa, which can be detected using the method of polyacrylamide gel electrophoresis (PAGE) followed by silver staining or Coomassie blue staining. Pure AAV should display only three major protein bands, such as shown in Fig 3.
Technical Documents
1. For further information about AAV administration and transduction results, please see the 
AAV(adeno associated virus) User Manual.
2. For more information about how to use in vivo Autophagy Flux Detection with AAV vectors, please refer to the AAV-LC3 Autophagy Flux Detection Manual.
AAV(adeno associated virus) User Manual.
2. For more information about how to use in vivo Autophagy Flux Detection with AAV vectors, please refer to the AAV-LC3 Autophagy Flux Detection Manual. Frequently Asked Questions(FAQs)
Reference
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