Ad-GFP-LC3
Cat:GM-AdVPAU02
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Description
Catalog No.
GM-AdVPAU02
Product Name
Ad-GFP-LC3
Production Scale
AAV packaging Serotype:
Final Deliverables
Lead Time(workday)
Quantity/Unit
Vials
Form
Frozen form
Shipping and Storage Guidelines
Shipped by dry ice, stored at -80 ℃, effective for 1 year. Avoid repeatedly freezing and thawing
Advantages
1. Autophagy flux detection in real time, quantitative.
2. High resolution, more accuracy and sensitivity than traditional approaches.
3. Broad range of host. Adv has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.
4. High infection efficiency. Almost 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection.
5. Great packaging capacity, up to 8kb.
6. Without integration. No alteration to host genome.
7. High titer. Up to 1011 pfu/ml.
Applications and Figures
Quality control description
Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.
Technical Documents
1. For further information about AAV administration and transduction results, please see the 
AAV(adeno associated virus) User Manual.
2. For more information about how to use in vivo Autophagy Flux Detection with AAV vectors, please refer to the AAV-LC3 Autophagy Flux Detection Manual.
AAV(adeno associated virus) User Manual.
2. For more information about how to use in vivo Autophagy Flux Detection with AAV vectors, please refer to the AAV-LC3 Autophagy Flux Detection Manual. Frequently Asked Questions(FAQs)
Reference
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2.Yuan Y, Y Zheng, X Zhang, Y Chen, X Wu, J Wu, Z Shen, L Jiang, L Wang, W Yang, J Luo, Z Qin, W Hu and Z Chen. (2017). BNIP3L/NIX-mediated mitophagy protects against ischemic brain injury independent of PARK2. Autophagy 13:1754-1766.
3.Yu T, F Guo, Y Yu, T Sun, D Ma, J Han, Y Qian, I Kryczek, D Sun, N Nagarsheth, Y Chen, H Chen, J Hong, W Zou and JY Fang. (2017). Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy. Cell 170:548-563 e16.
4.Shao BZ, P Ke, ZQ Xu, W Wei, MH Cheng, BZ Han, XW Chen, DF Su and C Liu. (2017). Autophagy Plays an Important Role in Anti-inflammatory Mechanisms Stimulated by Alpha7 Nicotinic Acetylcholine Receptor. Front Immunol 8:553.
5.Liu M, K Liang, J Zhen, M Zhou, X Wang, Z Wang, X Wei, Y Zhang, Y Sun, Z Zhou, H Su, C Zhang, N Li, C Gao, J Peng and F Yi. (2017). Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling. Nat Commun 8:413.
6.Chen ZH, WT Wang, W Huang, K Fang, YM Sun, SR Liu, XQ Luo and YQ Chen. (2017). The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway. Cell Death Differ 24:212-224.
7.Zhang Y, K Shen, Y Bai, X Lv, R Huang, W Zhang, J Chao, LK Nguyen, J Hua, G Gan, G Hu and H Yao. (2016). Mir143-BBC3 cascade reduces microglial survival via interplay between apoptosis and autophagy: Implications for methamphetamine-mediated neurotoxicity. Autophagy 12:1538-59.
8.Luo T, J Fu, A Xu, B Su, Y Ren, N Li, J Zhu, X Zhao, R Dai, J Cao, B Wang, W Qin, J Jiang, J Li, M Wu, G Feng, Y Chen and H Wang. (2016). PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7
9.Hua F, K Li, JJ Yu, XX Lv, J Yan, XW Zhang, W Sun, H Lin, S Shang, F Wang, B Cui, R Mu, B Huang, JD Jiang and ZW Hu. (2015). TRB3 links insulin/IGF to tumour promotion by interacting with p62 and impeding autophagic/proteasomal degradations. Nat Commun 6:7951.
10.Zhang X, Y Yuan, L Jiang, J Zhang, J Gao, Z Shen, Y Zheng, T Deng, H Yan, W Li, WW Hou, J Lu, Y Shen, H Dai, WW Hu, Z Zhang and Z Chen. (2014). Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury: Involvement of PARK2-dependent mitophagy. Autophagy 10:1801-13.
