Custom Lentivirus Production Service
Begin your customized Lentivirus production process
Introduction to Pre-made Lentivirus Production Service
Lentivirus is a powerful tool for delivering target genes into almost all types of mammalian cells in vitro and in vivo. In contrast to retroviruses, lentiviruses are imported much more actively into the nuclei of non-dividing cells and are stably integrated into the host cells’ genome independent of cell cycle. Compared to adenovirus and adeno-associated virus (AAV), HIV-based lentiviruses are much less cell toxic, less immunogenicity, and have better transduction efficiency in many cell types, and therefore hold unique promise as gene transfer agents. Genemedi engineered transfer and packaging lentivectors offer the highest lentiviral titers available along with a wide range of selection features including a broad variety of antibiotic selection markers and fluorescent-antibiotic fusion markers for real-time transduction monitoring.
Genemedi launched more than 10 thousand transgene plasmids, which have been sequenced to guarantee the right sequence. These premade plasmid pool are available for direct lentivirus packaging and may greatly shorten the supply period of lentivirus for more than 2 weeks.
Applications for Pre-made Lentivirus:
– Delivery of expression into hard-to-transfect cell types, such as neuronal cells, without using any transfection reagents.
– Highly reproducible and controllable expression delivery method.
– Creation of stable cell lines for long-term, high-level expression.
– Expression of genes in primary or drug-arrested cells.
– Creation of transgenic animals.
– Sub-cellular localization analysis by organelle targeting.
Properties
| Lentivirus from premade plasmid | |
|---|---|
| Quantity/Unit | Vials |
| Form | Frozen form |
| Suitable Types of Infection | In vivo infection in animals |
| Sipping and Storage Guidelines | Shipped by dry ice, stored at -80 ℃, effective for 1 year. Avoid repeatedly freezing and thawing. |
| Titer | > 1*10^8 TU/ml. |
Advantages
1. Provide a ready-to-use, easy delivery method for specific target and shrna expression.
2. Free of the often troublesome lentivector contraction and lentiviral virus production.
3. Consistent and reliable titers delivered at or above specified titer levels.
4. Fast turnaround time.
5. Safe-to-use (self-inactivating) lentiviral particles can deliver your gene into a wide range of cell lines including non-dividing, primary or stem cells.
6. Expert technical support, full confidentiality, and on-time delivery with all projects completed on-site.
Quality control description
Our optimized production of lentiviral vector and strict quality control systems supply customers with a high titer of functional recombinant lentiviral vectors. Two methods are employed to determine viral titers: physical titer (VP/mL) and functional titer (TU/mL). Physical titer is calculated by the level of protein, such as p24, or viral nucleic acid. The functional titer, a calculation of the active virus that can infect cells, is much less than the physical titer (100-1000 fold lower). The method we adopted is functional titer, which is an accurate solution for testing virus accurate activity and MOI. The physical titer can only reflect the number of virus particles, but not reflect the true viral activity, which will cause a large error in subsequent infection experiments.
Technical Documents
Lentivirus User Manual. Reference
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