LipoGeneTM transfection reagent
Cat:R-LG
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Introduction to LipoGeneTM transfection reagent
LipoGene™ transfection reagent is highly potent cationic lipofection reagent that has been shown to effectively transfect plasmids or siRNA, as well as nucleic acid-protein complexes, into cultured adherent and suspension cell lines. Researchers use LipoGenetm Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.
Transfection Protocol
Properties
Quantity/Unit
1 Vial.
Form
Liquid.
Sipping and Storage Guidelines
Shipped at 4°C, stored at 4 ° C, effective for 1year (do not freeze).
Suitable Cell Type
Established cell lines, hard-to-transfect cells, primary cells, stem cells.
Sample Type
Plasmid DNA, RNAi plasmids (shRNA, microRNA), synthetic siRNA.
Serum Compatible
Yes.
Advantages
1. Superior performance for co-transfection of siRNA and plasmid DNA.2. Proven efficacy in the presence of serum—eliminates the need to change media following transfection.3. Showed no obvious cytotoxicity for most cells without changing the culture medium for 72 hours.4. Suitable for both adherent and suspension cells and can be used for screening for stable expression of cell lines.5. For gene silencing, LipoGeneTM’s high-efficiency transfections provide the high levels of gene knockdown needed to achieve convincing results.6. LipoGeneTM is very easy to use—simply mix with nucleic acid and add to cell culture.
