Crispr Knowledge base
CRISPR/Cas9 premade products
Cat No. | Products Name | Type of Crispr | Viral vector | Promoter | Fluorescent/Resistance | Tag | Order
|
|---|---|---|---|---|---|---|---|
Ad-CMV-spCas9 |
spCas9 |
Adenovirus |
CMV |
zsgreen |
FLAG |
||
Adv-CMV-spCas9-zsgreen |
spCas9 |
Adenovirus |
CMV |
EGFP |
FLAG |
||
Adv-CMV-spCas9-EGFP |
spCas9 |
Adenovirus |
CMV |
Null |
FLAG |
||
Adv-CMV-spCas9-mcherry |
spCas9 |
Adenovirus |
CMV |
mcherry |
FLAG |
||
spCas9 |
lentivirus |
CMV |
puromycin |
FLAG |
|||
spCas9 |
lentivirus |
CMV |
zsgreen |
FLAG |
|||
spCas9 |
lentivirus |
CMV |
EGFP |
FLAG |
|||
spCas9 |
lentivirus |
CMV |
mcherry |
FLAG |
|||
spCas9 |
lentivirus |
CBH |
puromycin |
FLAG |
|||
spCas9 |
lentivirus |
CBH |
zsgreen |
FLAG |
Custom made CRISPR/Cas9 service
Introduction and principles of CRISPR/Cas9/Cas12/Cas13/Cpf1 gene editing system
CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences derived from viruses that have previously infected the prokaryotes and has the ability to recognize invasive homologous DNA sequences from similar viruses.
What is CRISPR/Cas9 delivery system
CRISPR/Cas9 system can be delivered in vitro by the non-viral reagents (such as polymer, liposome, nano-particle, electroporation) or viral vectors (such as lentivirus, adenovirus and AAV) and in vivo by adenovirus or AAV vectors.
CRISPR/Cas9 system – gRNA design and validation -principle and protocol
The PAM sequence that SpCas9 recognizes is NGG (AGG, TGG, CGG, GGG), while SaCas9 recognizes NNGRRT (NNGAAT, NNGAGT, NNGGAT, NNGGGT) or NNGRRN. For sgRNA, the length is about 21 or 22 nucleotides.
