Blog | Gene Therapy Technical Articles
Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine- Landscape and Classification of vaccines
Here we give some basic information on vaccine classification, generation, and application, and make a brief review on the current status of COVID-19 vaccine and tumor vaccine development both in the clinical trial stage and pre-clinical stage.
Viral vector-based vaccines: AAV vector-based vaccines
Here we give some information on viral vetor-based vaccines and AAV related products and service, principle, immune responses induced by AAV-based vaccine, application, its advantages and disadvantage, and optimization strategies.
Viral vector-based vaccines: Adenovirus (AdV) vector-based vaccines
Here we give some information on ADV related products and service, principle, immune responses induced by AAV-based vaccine, application, its advantages and disadvantage, and Optimization strategies.
Viral vector-based vaccines: Lentiviral vector-based vaccines
Here we give some information on Lentivirus related products and service, principle, immune responses induced by AAV-based vaccine, application, its advantages and disadvantage, and Optimization strategies.
Comparison of commonly used viral vector-based vaccines
Here we give some information on the comparison of commonly used viral vector-based vaccines.
Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine- A landscape for vaccine technology against infectious disease, COVID-19 and tumor.
Here we give some information on the DNA-based vaccines.
Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine- A landscape for vaccine technology against infectious disease, COVID-19 and tumor.
Here we give some information on the RNA-based vaccines.
Vaccines development for COVID-19/SARS-CoV-2
Here we give some basic information on Coronavirus Disease 2019 and SARS-CoV-2, and the vaccines development for COVID-19/SARS-CoV-2.
Tumor/Cancer vaccines – Examples of vaccines against tumor
Here we give some information on tumor/cancer vaccines development both in the clinical trial stage and pre-clinical stage.
Comparison among several kinds of novel vaccines
Here we give some information the comparison among several kinds of novel vaccines.
Challenge of COVID-19 vaccine discovery & development
Here we give some basic information on efficacy evaluation solution and tools of COVID-19 vaccines and neutralized antibodies.
GeneMedi Efficacy Assay/Evaluation Solutions for COVID-19 Vaccines and Therapeutic antibodies against SARS-CoV2(2019nCoV)
To meet accumulated mutating of SARS-CoV-2, GeneMedi keeps continually developing novel solutions and tools for efficacy evaluation of COVID-19 vaccines (and neutralized antibodies) against novel mutant SARS-CoV-2 (2019nCoV) lineages.
COVID-19 Guidance and information collection on the new mutant variants
Here we give some information on the new mutant variants.
COVID-19 News and announcements collection on the new mutant variants
Here we give some information on COVID-19 News and announcements on the new mutant variants.
Pre-made gene ORF plasmids for 2019 nCoV (SARS2 coronavirus, COVID-19)
Here we give some basic information on Pre-made gene ORF plasmids for 2019 nCoV (SARS2 coronavirus, COVID-19).
Validation of SARS-CoV-2 (2019nCoV) codon-optimized (CO) Spike overexpression in HEK293 cell line by GeneMedi’s adenoviral and lentiviral vector
Here we give some information on validation of SARS-CoV-2 (2019nCoV) codon optimized (CO) Spike expression in HEK293 cell line by GeneMedi’s adenoviral vector (GMV-V-2019nCoV-053, GMV-V-2019nCoV-047) and lentiviral vector (GMV-V-2019nCoV-051) with Immunofluorescence (left) and Western Blot (right) by FLAG antibody.
SARS-CoV-2 (2019nCoV, novel Coronavirus) Spike protein, S1 Protein, Spike S1-NTD, Spike S1-CTD, Spike-RBD and Spike trimer protein.
Here we give some information on SARS-CoV-2 (2019nCoV, novel Coronavirus) Spike protein, S1 Protein, Spike S1-NTD, Spike S1-CTD, Spike-RBD and Spike trimer protein.
SARS-CoV-2 (2019nCoV, novel Coronavirus) Envelop protein (Coronavirus E protein)
Here we give some information about SARS-CoV-2 (2019nCoV, novel Coronavirus) Envelop protein, membrane protein, Nucleocapsid protein, Non-structure protein, and COVID-19 pandemic, Coronavirus (Coronavirus) and genome of SARS-CoV-2 (2019nCoV).
SARS-CoV-2 (2019nCoV, novel Coronavirus) membrane protein (Coronavirus M protein)
Here we give some information about SARS-CoV-2 (2019nCoV, novel Coronavirus) membrane protein (Coronavirus M protein) and Nucleocapsid protein (Coronavirus N protein).
SARS-CoV-2 (2019nCoV, novel Coronavirus) Nucleocapsid protein (Coronavirus N protein)
Here we give some information about SARS-CoV-2 (2019nCoV, novel Coronavirus) Nucleocapsid protein (Coronavirus N protein).
SARS-CoV-2 (2019nCoV, novel Coronavirus)Non-structure protein (Nsp1-Nsp16)
Here we give some information about SARS-CoV-2 (2019nCoV, novel Coronavirus)Non-structure protein (Nsp1-Nsp16).
COVID-19 pandemic, Coronavirus (Coronavirus) and genome of SARS-CoV-2 (2019nCoV)
Here we give some information about COVID-19 pandemic, Coronavirus (Coronavirus) and genome of SARS-CoV-2 (2019nCoV).
Background of Coronavirus Disease 2019 (COVID-19, 2019-nCoV)
Here we give some information about the background of Coronavirus Disease 2019 (COVID-19, 2019-nCoV).
RT-PCR (Real time -PCR) in Coronavirus Disease 2019 (COVID-19) test
Here we make a brief introduction of RT-PCR (Real time -PCR) in Coronavirus Disease 2019 (COVID-19) test.
Immunoassay in Coronavirus Disease 2019 (COVID-19) test
Here we make a brief introduction of immunoassay in Coronavirus Disease 2019 (COVID-19) test.
CRISPR-based tools for Coronavirus Disease 2019 (COVID-19) test
Here we make a brief introduction of CRISPR-based tools for Coronavirus Disease 2019 (COVID-19) test.
Comparison of PCR vs Immunoassay vs Crispr-Based test
Here we give some information on comparison of PCR, Immunoassay and Crispr-Based test.
Introduction of Cre-loxP system
Here we make a brief introduction of Cre-loxP system.
Inducible-tissue specific Cre-loxP system for in vivo and in vitro study
Here we make a brief introduction of nducible-tissue specific Cre-loxP system for in vivo and in vitro study.
Tissue-specific AAV-Cre for in vivo study
Here we make a brief introduction of Inducible-tissue specific Cre-loxP system for in vivo and in vitro study.
Tissue/cell-specific Ad-Cre for in vivo or in vitro study
Here we make a brief introduction of tissue/cell-specific Ad-Cre for in vivo or in vitro study.
Principle of Cre-DIO system
Here we briefly introduce Principle of Cre-DIO.
Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression
Here we briefly introduce Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression.
AAV-DIO in neuroscience
Here we briefly introduce AAV-DIO in neuroscience.
Introduction of animal infectious disease
Here we make a brief introduction of animal infectious disease.
Lateral flow assays – strategies used in diagnosis of animal infectious disease for animal health
Here we make a brief introduction of lateral flow assays that used in diagnosis of animal infectious disease for animal health.
Enzyme-Linked Immunosorbent Assays (ELISA) – strategies used in diagnosis of animal infectious disease for animal health
Here we make a brief introduction of Enzyme-Linked Immunosorbent Assays (ELISA) that used in diagnosis of animal infectious disease for animal health.
Immunofluorescence staining (IF) – strategies used in diagnosis of animal infectious disease for animal health
Here we make a brief introduction of immunofluorescence staining (IF) that used in diagnosis of animal infectious disease for animal health.
Immunohistochemistry (IHC), In situ hybridization (ISH), Virus isolation (VI) – strategies used in diagnosis of animal infectious disease for animal health
Here we make a brief introduction of immunohistochemistry (IHC), In situ hybridization (ISH) and Virus isolation (VI) that used in diagnosis of animal infectious disease for animal health.
Electron microscopy (EM), Molecular Methods – strategies used in diagnosis of animal infectious disease for animal health
Here we make a brief introduction of electron microscopy (EM), Molecular Methods that used in diagnosis of animal infectious disease for animal health.
The strategies used in diagnosis of animal infectious disease for animal health
Here we discuss different diagnostic methods available to identify animal diseases and zoonotic infections.
Introduction of human infectious diseases caused by living pathogens
Here we make a brief introduction of human infectious diseases caused by living pathogens.
The strategies used in diagnosis of human Infectious diseases
Here we introduce the insights and detection methods of infectious disease, aiming to provide some helps for clinical diagnosis as well as epidemic prevention and control of infectious diseases.
Molecular Methods – strategie used in diagnosis of human Infectious diseases
Here we make a brief introduction of Molecular Methods that used in diagnosis of human Infectious diseases.
Immuno-assays – strategie used in diagnosis of human Infectious diseases
Here we make a brief introduction of immuno-assays that used in diagnosis of human Infectious diseases.
Viral Culture & Immunofluorescence (IF) Assay – strategies used in diagnosis of human Infectious diseases
Here we make a brief introduction of Viral Culture & Immunofluorescence (IF) Assay that used in diagnosis of human Infectious diseases.
Immunoblotting (WB) & Transmission Electron Microscopy (TEM) – strategies used in diagnosis of human Infectious diseases
Here we make a brief introduction of Immunoblotting (WB) & Transmission Electron Microscopy (TEM) that used in diagnosis of human Infectious diseases.
Codon Table Tool
This online tool shows standard genetic code, and also genetic codes for vertebrate mitochondrial, invertebrate mitochondrial, and other lower animals mitochondria or nuclear.
Nucleic Acids and Protein Calculation Conversions
Nucleic Acids and Protein Calculation Conversions, such as Extinction coefficient of deoxyribonucleoside triphosphate (dNTP) (pH7.0), Nucleic Acids ←→ Protein, Nucleic Acids, Spectrophotometric Conversions, Formulas for DNA Molar Conversions, Nucleic acid data and so on.
A alternative link when Sci-hub is not working
Recently, many readers feel paper prices cannot be afforded, Sci-Hub is a free download this artifact but cannot log in any longer. Will you let them bite people who live it literature? Do not worry! We have found a secret, just to be patient.
Introduction of Kozak sequence
Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI).
Cre-loxp system and Viral vector (AAV and adenovirus)-based Cre tools (AAV-Cre and Ad-Cre)
Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI).
Products collection for COVID-19 vaccines and drug discovery (neutralizing antibodies, peptides inhibitors or small molecule compounds)
High-throughput screening (HTS) assay development for neutralizing antibodies, peptides, small molecule compounds against COVID-19
Viral vector-based vaccine; DNA-based vaccine; RNA based vaccine – A landscape for vaccine technology against infectious disease, COVID-19 and tumor.
Here we give some basic information on vaccine classification, generation, and application, and make a brief review on the current status of COVID-19 vaccine and tumor vaccine development both in the clinical trial stage and pre-clinical stage.
Landscape Coronavirus Disease 2019 test (COVID-19 test) in vitro — A comparison of PCR vs Immunoassay vs Crispr-Based test
The outbreak of COVID-19, caused by 2019 novel coronavirus (2019-nCoV), has been a global public health threat and caught the worldwide concern. Scientists throughout the world are sparing all efforts to explore strategies for the determination of the 2019-nCoV virus and diagnosis of COVID-19 rapidly. Several assays are developed for COVID-19 test , including RT-PCR, coronavirus antigens-based immunoassays, and CRISPR-based strategies (Cas13a or Cas12a), etc.
Collection of COVID-19 knonwledge base
GeneMedi produces kinds of recombinant 2019 nCoV(SARS2 coronavirus) antigens as shown below: Nucleocapsid(N protein), Spike(S protein), RBD, S1+S2 ECD, Envelope (E protein), 3C-like Proteinase. All the recombinant proteins of 2019 nCoV are suitable for in functional ELISA,lateral-flow tests, and other immunoassays.
An Insight of comparison between COVID-19 (2019-nCoV) and SARS-CoV in pathology and pathogenesis
COVID-19, a novel pathogenic coronavirus emerged in China and spread globally rapidly. COVID-19 shares above 85% identity in genome with SARS-CoV. Patients infected by COVID-19 and SARS-CoV also reveal similar clinical characteristics. Here we compare the clinical and pathological features between patients infected by COVID-19 and SARS-CoV respectively.
Background of 2019 Wuhan Novel coronavirus – From Wiki
2019 Wuhan Novel coronavirus, (2019-nCoV by WHO), is also known as Wuhan coronavirus, Wuhan seafood market pneumonia virus and Wuhan pneumonia, is a positive-sense, single-stranded RNA coronavirus first reported in 2019. The virus was genomically sequenced after nucleic acid testing on a positive patient sample in a patient with pneumonia during the 2019–2020 Wuhan pneumonia outbreak.
Oncolytic virus immunotherapy (HSV, oncolytic adenovirus) and cancer therapy
Oncolytic virotherapy is a novel therapeutic modality utilizing oncolytic viruses (such as herpes simplex-, pox-, parvo-, or adenoviruses), which selectively replicate in cancer cells, to directly induce tumor cell lysis or indirectly reactivate human immune system to mediate tumor destruction.
CAR-T immunotherapy and cancer therapy
Chimeric antigen receptor (CAR)-modified T cells (CAR-T) are T cells genetically engineered to express CAR, which can specifically recognize their target antigen via the scFv binding domain of CAR, resulting in T cell activation to specifically target and destroy tumor cells.
TCR-T immunotherapy and cancer therapy
T-cell receptor (TCR)–engineered T-cell therapy (TCR-T) is one potentially powerful treatment that utilizes genetically modified natural T cells to specifically target tumors and destroy tumors with greater potentials.
CAR-NK immunotherapy and cancer therapy
Although CAR-T has been applied in clinic, quite a lot of side effects, such as off-target, cytokine release syndrome (CRS) etc., pose a great restriction in their further clinical application.
Immuno-oncology cell therapy
In the past several decades, great progress has been made in the field of anti-tumor therapy, but there are also multiple strategies for tumors to evade the recognition and efficient suppression by the immune system. Therefore, a variety of immunotherapeutic targets and strategies have been developed to reactivate and reorganize the human immune system, such as CAR-T, TCR-T, CAR-NK, DC-CIK, oncolytic virus and so on.
Stem Cell Therapy
Cell therapy based on stem cells is a kind of regenerative medicine with great potential for the treatment of diseases via the differentiation ability or the paracrine effects of stem cells as well as their derivatives.
Genetic re-engineering cell therapy
Besides the immune-oncology therapy and stem cell derived therapy, gene replacement therapy via lentivirus, adenovirus and adenovirus associated virus (AAV) as well as genetic engineered strategies via CRISPR, ZLN, TALEN have been widely applied in the field of cell therapy.
How much do you trust Dr. Google?
Women experiencing signs of breast cancer vary in how they value, use, and trust ‘Dr. Google’ when making sense of their symptoms, a new study reports.
Weather Forecasting
Weather forecasting is the application of current technology and science to predict the state of the atmosphere for a future time and a given location.
Amino Acid Structure Chart and Reference Table
Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains.
Amino Acid Codes
Amino acids are the backbone of peptides and proteins. All amino acids contain both amino and carboxylic acids, and in certain cases, side chains.
